| 产品编号 | bs-22470R |
| 英文名称 | Haspin Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶蛋白抗体 |
| 别 名 | germ cell associated 2; Germ cell-specific gene 2 protein; GSG2; H-Haspin; Haploid germ cell-specific nuclear protein kinase; HASP_HUMAN; Serine/threonine-protein kinase Haspin. |
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Specific References (3) | bs-22470R has been referenced in 3 publications.
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[IF=3.329] Zhu D et al. HASPIN is involved in the progression of gallbladder carcinoma. Exp Cell Res. 2020 Jan 24:111863. IHC-P ; Human. 222
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[IF=3.329] Han X et al. Haspin knockdown can inhibit progression and development of pancreatic cancer in vitro and vivo. Exp Cell Res. 2019 Sep 4:111605. IHC-P ; Human. 222
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[IF=3.322] Luhao Li. et al. GSG2 promotes tumor growth through regulating cell proliferation in hepatocellular carcinoma. BIOCHEM BIOPH RES CO. 2022 Oct;625:109 IHC ; Human. 222
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| 研究领域 | 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, ) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 88 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Haspin: 421-520/754 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Haspins is a protein family containing a distinctive C-terminal kinase domain and a divergent N-terminus. Haspin homologues occur within a diverse group of eukaryotes, including animals, plants and fungi, which suggests an early evolutionary origin. Haspin, a nuclear protein strongly expressed in male germ cells, is responsible for the phosphorylation of histone H3 at Thr-3. Depletion of Haspin RNA prevents normal alignment of chromosomes at metaphase, suggesting a crucial role for haspin during chromosome segregation. Expression of haspin also occurs in adult thymus and bone marrow, with weaker expression in adult prostate, intestine, lung, spleen, and lymph node. The gene encoding human haspin maps to chromosome 17p13. Function: Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. Subcellular Location: Nucleus. Chromosome. Cytoplasm, cytoskeleton, spindle. Note=Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis. Tissue Specificity: Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines. Post-translational modifications: Autophosphorylated on both serine and threonine residues (By similarity). Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 83903 Human Omim: 609240 Human SwissProt: Q8TF76 Human Unigene: 534059 Human |
| 产品图片 |
Sample:
Thymus(Rat) Lysate at 40 ug
Kidney(Rat) Lysate at 40 ug
Kidney(Mouse) Lysate at 40 ug
Primary: Anti-Haspin (bs-22470R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
