| 产品编号 | bs-48001P |
| 英文名称 | Recombinant human IL-1b protein (Active, HEK293) |
| 中文名称 | 重组人白细胞介素-1β (活性的, Tag free) |
| 别 名 | Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta; Recombinant human IL-1b protein (Active, HEK293) IL-1 beta 重组蛋白; 白细胞介素-1β; 重组人白细胞介素-1β; |
| 克 隆 号 | |
| 理论分子量 | kDa |
| 检测分子量 | 18-25 |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 117-269/269 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
IL-1 includes two pleiotropic cytokines, IL1α and IL-1β. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. This recombinant human IL-1 beta is produced by human cells. Biological activity: The activity was measured by its bility to stimulate the proliferation of the proliferation of mouse D10S cells. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 产品图片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
