| 产品编号 | D10213 |
| 英文名称 | Pyruvate Kinase from rabbit muscle |
| 中文名称 | 丙酮酸激酶 |
| 别 名 | EC2.7.1.40; Pyruvate Kinase; PK; Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600 units/mg protein; ATP:pyruvate 2-O-phosphotransferase; 丙酮酸激酶 (EC2.7.1.40); 丙酮酸激酶(来源于家兔肌肉); 家兔肌肉丙酮酸激酶; |
| 克 隆 号 | |
| 保存条件 | Store at -20℃. |
| 产品介绍 |
CAS: 9001-59-6 Molecular Weight: 237kDa MDL: MFCD00081946 grade: BR purity: 99%+ source: rabbit muscle form：lyophilized powder type: Type III foreign activity: lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01% Unit Definition: One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37℃. Application: Pyruvate kinase is an enzyme involved in glycolysis and is involved in gluconeogenesis. It has been used in plant spectrophotometric assays to measure ATP hydrolysis. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency. Biochem/physiol Actions: Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa. Isoelectric Point: 7.6 Optimal pH: ~7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
