| 产品编号 | bs-22308R |
| 英文名称 | Phospho-HSD17B13 (Ser33) Rabbit pAb |
| 中文名称 | 磷酸化17β羟基类固醇脱氢酶13抗体 |
| 别 名 | HSD17B13(phospho S33); HSD17B13(phospho Ser33); phospho-HSD17B13(S33); p-HSD17B13(Ser33); 17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; DHB13_HUMAN; HMFN0376; Hsd17b13; SCDR9; Short-chain dehydrogenase/reductase 9; UNQ497/PRO1014. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 信号转导 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 Flow-Cyt=1ug/Test ICC=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human HSD17B13 around the phosphorylation site of Ser33 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
17 beta-HSD13 (17 beta hydroxysteroid dehydrogenase type 13), also designated Short-chain dehydrogenase/reductase 9 (SCDR9), belongs to the 17 beta-HSD family of proteins, which regulate the availability of steroids within various tissues throughout the body. 17 beta-HSD13 is a 300 amino acid secreted protein that is highly expressed in liver and is also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. The gene encoding 17 beta-HSD13 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. Subcellular Location: Secreted. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: Q7Z5P4 Gene ID: 345275 Database links: Entrez Gene: 345275 Human Entrez Gene: 243168 Mouse Omim: 612127 Human SwissProt: Q7Z5P4 Human SwissProt: Q8VCR2 Mouse Unigene: 284414 Human Unigene: 284944 Mouse Unigene: 25104 Rat |
| 产品图片 |
Sample:
Lane 1: Human U-2 OS cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human U937 cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-Phospho-HSD17B13 (Ser33) (bs-22308R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa
Blank control（black line）:HepG2.
Primary Antibody (green line): Rabbit Anti-Phospho-HSD17B13 (Ser33) antibody (bs-22308R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
