| 产品编号 | bs-22921R |
| 英文名称 | MRas Rabbit pAb |
| 中文名称 | 原癌基因M-ras抗体 |
| 别 名 | M-RAS; M ras; muscle RAS oncogene homolog precursor; Muscle and microspikes Ras; Muscle Ras oncogene homologue; R ras3; R-ras3; Ras related protein MRas; Ras related protein RRas3; RRas3; XRas. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse MRas: 201-259/259 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MRas is a member of the RAS superfamily of GTP-binding proteins which are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. MRas participates in reorganisation of actin cytoskeleton. It is also involved in focal adhesion and can activate MAPK signalling. Function: May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Tissue Specificity: Expression highly restricted to the brain and heart. Similarity: Belongs to the small GTPase superfamily. Ras family. SWISS: O08989 Gene ID: 17532 Database links: Entrez Gene: 22808 Human Entrez Gene: 17532 Mouse Omim: 608435 Human SwissProt: O14807 Human SwissProt: O08989 Mouse Unigene: 527021 Human Unigene: 2045 Mouse Unigene: 10641 Rat M-ras癌变基因的表达产物Ras蛋白存在于多数肿瘤之中，目前是肿瘤研究较重要的蛋白之一. |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Testis (Mouse) Lysate at 40 ug
Lane 4: Testis (Rat) Lysate at 40 ug
Lane 5: Pancreas (Rat) Lysate at 40 ug
Lane 6: Liver (Rat) Lysate at 40 ug
Lane 7: Kidney (Mouse) Lysate at 40 ug
Lane 8: Kidney (Rat) Lysate at 40 ug
Primary: Anti-MRas (bs-22921R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
