| 产品编号 | bsm-52841R |
| 英文名称 | Annexin V Recombinant Rabbit mAb |
| 中文名称 | 膜粘连蛋白5重组兔单抗 |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 6C7 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin V |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Subunit: Monomer. Binds ATRX and EIF5B. Interacts with hepatitis B virus (HBV). Subcellular Location: Monomer. Binds ATRX and EIF5B. Interacts with hepatitis B virus (HBV). Post-translational modifications: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. DISEASE: Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P08758 Gene ID: 308 Database links: Entrez Gene: 308 Human Entrez Gene: 11747 Mouse Omim: 131230 Human SwissProt: P08758 Human SwissProt: P48036 Mouse Unigene: 480653 Human Unigene: 1620 Mouse Unigene: 3318 Rat |
| 产品图片 |
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: Stomach (Mouse) Lysate at 40 ug
Lane 6: Lung (Rat) Lysate at 40 ug
Lane 7: Uterus (Rat) Lysate at 40 ug
Lane 8: Spleen (Rat) Lysate at 40 ug
Lane 9: Stomach (Rat) Lysate at 40 ug
Lane 10: HepG2 (Human) Cell Lysate at 30 ug
Lane 11: Panc-1 (Human) Cell Lysate at 30 ug
Lane 12: K562 (Human) Cell Lysate at 30 ug
Lane 13: Jurkat (Human) Cell Lysate at 30 ug
Lane 14: Huvec(Human) Cell Lysate at 30 ug
Primary:
Anti-Annexin V (bsm-52841R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 34 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
