| 产品编号 | C7102 |
| 英文名称 | HAT Supplement (50×) |
| 中文名称 | HAT培养基添加剂 (50×) |
| 别 名 | HAT Media Supplement (50×) Hybri-Max™; H0262 (Sigma-Aldrich); HAT Media Supplement(50×); HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement HAT补充剂; 次黄嘌呤氨基蝶呤胸苷（HAT）培养基补充剂; HAT添加剂; |
| 克 隆 号 | |
| 保存条件 | Store at -20℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
HAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma; Application: HAT Media Supplement (50×) Hybri-Max™ has been used: • in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones. • in the generation of mAbs (monoclonal antibodies). • in the luciferase-based bioassay (LUC) assay. Reconstitution: Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
