| 产品编号 | bsm-54301R |
| 英文名称 | Galectin 8 Recombinant Rabbit mAb |
| 中文名称 | 半乳糖凝集素8重组兔单抗 |
| 别 名 | Gal-8; PCTA-1; PCTA1; Po66-CBP; 1200015E08Rik; D13Ertd524e; Lgals-8; LEG8_HUMAN; LGALS8; Po66 carbohydrate-binding protein (Po66-CBP); Prostate carcinoma tumor antigen 1 (PCTA-1); LEG8_MOUSE; LEG8_RAT; 30 kDa S-type lectin; RL-30; |
| 研究领域 | 肿瘤 免疫学 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 1A4 |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-1000 IP=1:20-100 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:400-800 IF=1:400-800 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Galectin 8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy. Similarity: Contains 2 galectin domains. SWISS: O00214 Gene ID: 3964 Database links: Entrez Gene: 3964 Human Entrez Gene: 56048 Mouse Omim: 606099 Human SwissProt: O00214 Human SwissProt: Q9JL15 Mouse Unigene: 4082 Human Unigene: 708114 Human Unigene: 448495 Mouse Unigene: 474138 Mouse Unigene: 10069 Rat |
| 产品图片 |
4% Paraformaldehyde-fixed PANC-1 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Galectin 8) monoclonal Antibody, unconjugated (bsm-54301R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The PANC-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Galectin 8 antibody (bsm-54301R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
