| 产品编号 | bsm-54301R |
| 英文名称 | Galectin 8 Recombinant Rabbit mAb |
| 中文名称 | 半乳糖凝集素8重组兔单抗 |
| 别 名 | Gal 8; Gal-8; Gal8; Galectin-8; LEG8_HUMAN; LGAL S8; LGALS8; PCTA 1; PCTA-1; PCTA1; Po66 carbohydrate binding protein; Po66 carbohydrate-binding protein; Po66 CBP; Po66-CBP; Prostate carcinoma tumor antigen 1. |
| 研究领域 | 肿瘤 免疫学 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 1A4 |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-1000 IP=1:20-100 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:400-800 IF=1:400-800 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Galectin 8 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy. Similarity: Contains 2 galectin domains. SWISS: O00214 Gene ID: 3964 Database links: Entrez Gene: 3964 Human Entrez Gene: 56048 Mouse Omim: 606099 Human SwissProt: O00214 Human SwissProt: Q9JL15 Mouse Unigene: 4082 Human Unigene: 708114 Human Unigene: 448495 Mouse Unigene: 474138 Mouse Unigene: 10069 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 8) Monoclonal Antibody, Unconjugated (bsm-54301R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
