| 产品编号 | bs-48018P |
| 英文名称 | Recombinant human TGF-Beta 1 protein (Active, HEK293) |
| 中文名称 | 重组人TGF β-1 (活性的, Tag free) |
| 别 名 | rhTGFβ-1; CED; DPD1; LAP; Latency-associated peptide; TGFβ-1; TGFβ1; TGF beta; TGF-beta 1; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1; Recombinant human TGF-Beta 1 protein (Active, HEK293) 人转化生长因子B 1重组蛋白; 人转化生长因子β 1重组蛋白; 重组人转化生长因子β 1; 人重组人转化生长因子β 1; 重组人TGF β-1; |
| 克 隆 号 | |
| 理论分子量 | 25 kDa |
| 检测分子量 | 25 |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 279-390/390 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Transforming growth factor beta 1 (TGF-β1) is one of three closely related mammalian members of the large TGF-β superfamily, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. TGF-β1 is the most abundant isoform secreted by almost every cell type. TGF-β1 is a multifunctional cytokine that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGF-β1 and have specific receptors for it. TGF-β1 positively and negatively regulates many other growth factors. TGF-β1 plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and ifferentiation in committed osteoblasts. This recombinant human TGF-β is produced by human cells.(Tag free) Biological activity: The activity was measured by its ability to inhibit the IL-4 induced proliferation in mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 产品图片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
