| 产品编号 | bs-48024P |
| 英文名称 | Recombinant human G-CSF protein (Active, CHO) |
| 中文名称 | 重组人G-CSF (活性的, Tag free) |
| 别 名 | rhG-CSF; GCSF; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; Csfg; Filgrastim; GCSA; Granulocyte colony stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin; Granulocyte Colony Stimulating Factors 3; CSF3_HUMAN ; 人G-CSF重组蛋白; 重组人G-CSF; 人重组G-CSF; 重组人G-CSF; 粒细胞集落刺激因子; 集落刺激因子; 粒细胞生长因子; 重组人粒细胞集落刺激因子; |
| 克 隆 号 | |
| 理论分子量 | kDa |
| 检测分子量 | 21-25 |
| 性 状 | Lyophilized |
| 浓 度 | ＞0.5 mg/ml |
| 物 种 | Human |
| 序 列 | 31-204/207 |
| 纯 度 | >95% as determined by SDS-PAGE |
| 纯化方法 | AC |
| 内毒素 | <0.5 EU/μg as determined by LAL. |
| 表达系统 | HEK293 cell |
| 活性 | Yes |
| 标签 | Tag free |
| 缓 冲 液 | 0.22μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH7.4 |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor belonging to the CSF family. G-CSF is mainly expressed in monocyte/macrophage lineage. It is also expressed in cells of mesodermal origin, including vascular endothelial cells, fibroblasts, and mesothelial cells. G-CSF acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This recombinant human G-CSF is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line). Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 产品图片 |
Measured in a cell proliferation assay using of murine NFS-60 cells is < 0.5 ng/ml, corresponding to a specific Bioactivity of ≥ 1 x 10^7 units/mg.
The purity of the protein is greater than 95% as determined by reducing SDS-PAGE.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
