| 产品编号 | bsm-52822R |
| 英文名称 | HMGCR Recombinant Rabbit mAb |
| 中文名称 | HMGCR重组兔单抗 |
| 别 名 | 3 hydroxy 3 methylglutaryl CoA reductase; 3 hydroxy 3 methylglutaryl Coenzyme A reductase; 3 hydroxymethylglutaryl CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase(NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; Hydroxymethylglutaryl CoA reductase; LDLCQ3; MGC103269. |
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Specific References (6) | bsm-52822R has been referenced in 6 publications.
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[IF=7.422] Han Zeng. et al. CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing. Mol Metab. 2021 Dec;:101428 WB,IP ; Mouse,Human. 222
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[IF=6.317] Mubai Sun. et al. Black bean husk and black rice anthocyanin extracts modulated gut microbiota and serum metabolites for improvement in type 2 diabetic rats. FOOD FUNCT. 2022 Jun;: WB ; Rat. 222
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[IF=4.081] Huo Yihui. et al. Effect of Copper Exposure on the Cholesterol Metabolism in Broiler Liver. BIOL TRACE ELEM RES. 2023 Mar;:1-9 WB ; Chicken. 222
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[IF=2.838] Min-Ho Lee. et al. Ethanol Extract of Pinus koraiensis Leaves Mitigates High Fructose-Induced Hepatic Triglyceride Accumulation and Hypertriglyceridemia. APPL SCI-BASEL. 2022 Jan;12(13):6745 WB ; Human, Rat. 222
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[IF=2.65] Huiming Hu. et al. The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats.. EVID-BASED COMPL ALT. 2022 Nov;2022:3033311-3033311 WB ; Rat. 222
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[IF=2.629] Mei Hui. et al. The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evid-Based Compl Alt. 2021;2021:3155266 WB ; Rat. 222
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 7C5 |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:1000-2000 IP=1:10-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human HMGCR: 400-500 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Function: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis. Subunit: Homodimer. Subcellular Location: Endoplasmic reticulum membrane. Peroxisome membrane. Similarity: Belongs to the HMG-CoA reductase family. SWISS: P04035 Gene ID: 3156 Database links: Entrez Gene: 3156 Human Entrez Gene: 15357 Mouse Omim: 142910 Human SwissProt: P04035 Human SwissProt: Q01237 Mouse Unigene: 628096 Human Unigene: 643495 Human Unigene: 316652 Mouse Unigene: 9437 Rat |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with HMGCR monoclonal antibody, unconjugated (bsm-52822R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
