| 产品编号 | bsm-52273R |
| 英文名称 | MERTK Recombinant Rabbit mAb |
| 中文名称 | MERTK重组兔单抗 |
| 别 名 | MERTK_HUMAN; Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; MER; MER proto-oncogene, tyrosine kinase; RP38; c-Eyk; c-mer; Tyro12. |
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Specific References (1) | bsm-52273R has been referenced in 1 publications.
111
[IF=3.641] Arshad Zahoor et al. MerTK negatively regulates Staphylococcus aureus induced inflammatory response via Toll-like receptor signaling in the mammary gland. Mol Immunol
. 2020 Apr 2;122:1-12. WB ; mouse. 222
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| 研究领域 | 肿瘤 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 1C11 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IP=1:10-50 IHC-P=1:50-200 IHC-F=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 108 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human MERTK: 25-48 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. Peptide-transporters 2 [solute carrier family 15 (H+/peptide transporter), member 2; SLC15A2; PEPT2 ; Oligopeptide transporter, kidney isoform ; Kidney H(+)/peptide cotransporter; ]. Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Subunit: Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets. Post-translational modifications: Autophosphorylated on Tyr-744, Tyr-748 and Tyr-749 in the activation loop allowing full activity. Autophosphorylated on Tyr-867 leading to recruitment of downstream partners of the signaling cascade such as PLCG2. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. SWISS: Q12866 Gene ID: 10461 Database links: Entrez Gene: 10461 Human Omim: 604705 Human SwissProt: Q12866 Human Unigene: 306178 Human |
| 产品图片 |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-MERTK (bsm-52273R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 180 kD
Observed band size: 180 kD
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MERTK Monoclonal Antibody, Unconjugated(bsm-52273R) at 1:150 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
