| 产品编号 | bsm-52386R |
| 英文名称 | CD3 epsilon Recombinant Rabbit mAb |
| 中文名称 | CD3E重组兔单抗 |
| 别 名 | CD3epsilon; IMD18; T3E; TCRE; CD3; CD3E_HUMAN; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E_MOUSE; |
 |
Specific References (2) | bsm-52386R has been referenced in 2 publications.
111
[IF=3.549] Jingjing Li. et al. Circular RNA hsa_circ_0068252 functions in cisplatin resistance and immune response via miR-1304-5p/PD-L1 axis in non-small cell lung cancer. CHEMOTHERAPY. ;: IA ; Human. 222
111
[IF=3.098] Junli Zhang. et al. KCNQ1OT1 contributes to sorafenib resistance and programmed death‑ligand‑1‑mediated immune escape via sponging miR‑506 in hepatocellular carcinoma cells. Int J Mol Med. 2020 Nov;46(5):1794-1804 ELISA ; Human. 222
|
| 研究领域 | 肿瘤 细胞生物 免疫学 干细胞 细胞膜受体 细胞表面分子 淋巴细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 7A4 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IP=1:20-50 IHC-P=1:100-500 IHC-F=1:50-200 Flow-Cyt=1ug/Test （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | 23 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CD3 epsilon |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. Function: The CD3 complex mediates signal transduction. Subunit: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain. SWISS: P07766 Gene ID: 916 Database links: Entrez Gene: 916 Human Entrez Gene: 12501 Mouse Omim: 186830 Human SwissProt: P07766 Human SwissProt: P22646 Mouse Unigene: 3003 Human Unigene: 210361 Mouse |
| 产品图片 |
Sample:
Lane 1: Molt-4 (Human) Cell Lysate at 30 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Primary: Anti-CD3 epsilon (bsm-52386R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (Human gastric cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (bsm-52386R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (bsm-52386R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (bsm-52386R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
