| 产品编号 | bsm-51535M |
| 英文名称 | C3 Mouse mAb |
| 中文名称 | 补体C3单克隆抗体 |
| 别 名 | Acylation stimulating protein cleavage product; Acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody C3 antibody C3a anaphylatoxin; CO3_HUMAN; Complement C3 alpha chain; Complement C3; Complement C3 beta chain; Complement C3 precursor; Complement C3b alpha chain; Complement C3c alpha' chain fragment 2; Complement C3c fragment; Complement C3d fragment; Complement C3f fragment; Complement C3g fragment; Complement component 3; Complement component 3 precursor; Complement component C3; Complement factor 3; CPAMD1; Plp. |
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Specific References (1) | bsm-51535M has been referenced in 1 publications.
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[IF=3.252] Ganggang Li. et al. Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells. Brain Res. 2022 Jan;1774:147723 WB,IF ; Rat. 222
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| 研究领域 | 免疫学 神经生物学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | B3N11 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 187 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C3: 1301-1400/1663 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. Subcellular Location: Secreted. Tissue Specificity: Plasma. The acylation stimulating protein (ASP) is expressed in adiopocytes and released into the plasma during both the fasting and postprandial periods. Similarity: Contains 1 anaphylatoxin-like domain. Contains 1 NTR domain. SWISS: P01024 Gene ID: 718 Database links: Entrez Gene: 718 Human SwissProt: P01024 Human |
| 产品图片 |
Sample:
Lane 1: Hela cell lysates
Lane 2: HepG2 cell lysates
Lane 3: K562 cell lysates
Lane 4: CCRF-CEM cell lysates
Lane 5: MDA-MB-453 cell lysates
Primary: Anti-C3 (bsm-51535M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 187 kD
Observed band size: 145 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
