| 产品编号 | bsm-51607M |
| 英文名称 | XIAP Mouse mAb |
| 中文名称 | X-连锁凋亡蛋白/性连锁凋亡抑制蛋白单克隆抗体 |
| 别 名 | X-linked Inhibitor of Apoptosis Protein; RIAP-3; Baculoviral IAP repeat-containing protein 4; E3 ubiquitin-protein ligase XIAP; Inhibitor of apoptosis protein 3; X-linked IAP; IAP-like protein; HILP; BIRC4; HILP; XLP2; ILP1; Xiap; MIHA; hILP; IAP3; API3; |
| 研究领域 | 肿瘤 细胞生物 细胞凋亡 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | D1G6 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IHC-P=1:20-100 IHC-F=1:20-100 ICC=1:25 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human XIAP |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011] Function: Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. Subunit: Monomer, and homodimer. Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of MAP3K7IP1 and AIFM1. Interacts with TCF25. Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous, except peripheral blood leukocytes. Post-translational modifications: Ubiquitinated and degraded by the proteasome in apoptotic cells. Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. DISEASE: Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. Similarity: Belongs to the IAP family. Contains 3 BIR repeats. Contains 1 RING-type zinc finger. SWISS: P98170 Gene ID: 331 Database links: Entrez Gene: 331 Human SwissProt: P98170 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with XIAP monoclonal antibody, unconjugated (bsm-51607M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human MOLT4 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human Raji cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human A549 cell lysates
Primary: Anti-XIAP (bsm-51607M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Paraformaldehyde-fixed, paraffin embedded (Human kidney section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (XIAP) Monoclonal Antibody, Unconjugated (bsm-51607M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
