| 产品编号 | bsm-51653M |
| 英文名称 | REG3G Mouse mAb |
| 中文名称 | REG3G单克隆抗体 |
| 别 名 | Reg III gamma; REG3G_HUMAN; Reg3 gamma; Reg3gamma; Reg-3gamma; Regenerating islet-derived protein 3-gamma; REG-3-gamma; Pancreatitis-associated protein 1B; PAP-1B; Pancreatitis-associated protein IB; PAP IB; Regenerating islet-derived protein III-gamma; Reg III-gamma. |
| 研究领域 | 免疫学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | F6D9 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-1000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 17 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human REG3G. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The regeneration (REG) family consists of secretory proteins involved in liver, pancreatic, gastric and intestinal cell proliferation or differentiation. Members of the REG family are divided into four subclasses, designated types I, II, III and IV, all of which share a common gene structure containing five introns and six exons. Members of the Reg family have been implicated in the regulation of cell growth, tumorigenesis and the progression of cancer. Reg lll(regenerating islet-derived 3 gamma), also known as pancreatitis-associated protein 1B, PAP1B, or UNQ429, is a 175 amino acid secreted protein that is expressed almost exclusively in pancreas, with low levels of expression in testis. Reg lll?functions as an antimicrobial protein involved in controlling bacterial proliferation and may be induced during acute pancreatitis. The gene encoding Reg lll?maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Function: Might be a stress protein involved in the control of bacterial proliferation. Subcellular Location: Secreted. Tissue Specificity: Expressed almost exclusively in the pancreas. Also expressed in testis. Not found in small intestine. Similarity: Contains 1 C-type lectin domain. SWISS: Q6UW15 Gene ID: 130120 Database links: Entrez Gene: 130120 Human SwissProt: Q6UW15 Human |
| 产品图片 |
Sample:
Lane 1: Human Pancreas tissue lysates
Primary: Anti-REG3G (bsm-51653M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreas tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (REG3G) Monoclonal Antibody, Unconjugated (bsm-51653M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
