| 产品编号 | bsm-51659M |
| 英文名称 | Hexokinase II Mouse mAb |
| 中文名称 | 己糖激酶2单克隆抗体 |
| 别 名 | Hexokinase II; Hexokinase 2 muscle; Hexokinase type II; Hexokinase-2; HK 2; HK II; HK2; HKII; HxK 2; HxK2; HXK2_HUMAN; Muscle form hexokinase; DKFZp686M1669. |
| 研究领域 | 肿瘤 心血管 染色质和核信号 信号转导 转录调节因子 新陈代谢 线粒体 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | S2G6 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-400 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 101 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HK2 (Hexokinase II) between 1-170 amino acids. |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. Function: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Subunit: Monomer. Subcellular Location: Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Similarity: Belongs to the hexokinase family. SWISS: P52789 Gene ID: 3099 Database links: Entrez Gene: 3099 Human SwissProt: P52789 Human |
| 产品图片 |
Sample:
Lane 1: Hela cell lysates
Lane 2: 293 cell lysates
Lane 3: Jurkat cell lysates
Primary: Anti-Hexokinase II (bsm-51659M) at 1/500~1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 101 kD
Observed band size: 101 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
