| 产品编号 | bsm-51697M |
| 英文名称 | PSMC2 Mouse mAb |
| 中文名称 | PSMC2单克隆抗体 |
| 别 名 | 26S protease regulatory subunit 7; PRS7_HUMAN; 26S protease complex; 26S proteasome AAA-ATPase subunit RPT1; mammalian suppressor of sgv-1 of yeast; MSS1; Nbla10058; protease 26S subunit 7; proteasome 26S ATPase subunit 2; Proteasome 26S subunit ATPase 2; proteasome 26S subunit ATPase 2; Protein MSS1; putative protein product of Nbla10058; S7 antibody. |
| 研究领域 | 细胞生物 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | G5K1 |
| 交叉反应 | Human, (predicted: Mouse, Cow, ) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PSMC2. |
| 亚 型 | IgG1,Κ |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2011] Function: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation. Subunit: Interacts with NDC80 and SQSTM1. Interacts with PAAF1. Interacts with HIV-1 Tat. Interacts with TRIM5. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, P-body. Note=Co-localizes with TRIM5 in the cytoplasmic bodies. Similarity: Belongs to the AAA ATPase family. SWISS: P35998 Gene ID: 5701 Database links: Entrez Gene: 5701 Human Entrez Gene: 19181 Mouse SwissProt: P35998 Human SwissProt: P46471 Mouse |
| 产品图片 |
Sample:
Lane 1: Jurkat cell lysates
Lane 2: U-2OS cell lysates
Lane 3: Hela cell lysates
Lane 4: MCF-7 cell lysates
Primary: Anti-PSMC2 (bsm-51697M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
