| 产品编号 | bsm-51708M |
| 英文名称 | PDCD1LG2 Mouse mAb |
| 中文名称 | 程序性死亡配体2（CD273）单克隆抗体 |
| 别 名 | PD L2; PD-L2; PD1L2_HUMAN; B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7 DC; Butyrophilin B7-DC; Butyrophilin B7DC; CD 273; CD273; CD273 antigen; MGC142238; MGC142240; PD 1 ligand 2; PD-1 ligand 2; PD1 ligand 2; PDCD 1 ligand 2; PDCD1 ligand 2; PDL 2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2. |
| 研究领域 | 免疫学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | G8F6 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:1000-2000 IHC-P=1:100-400 IHC-F=1:100-400 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDCD1LG2: 31-130/273 |
| 亚 型 | IgG1,Κ |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis. [provided by Alliance of Genome Resources, Nov 2021] Function: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Subunit: Interacts with PDCD1. Subcellular Location: Isoform 3: Secreted (Probable). Isoform 2: Endomembrane system; Single-pass type I membrane protein. Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. Post-translational modifications: Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9BQ51 Gene ID: 80380 Database links: Entrez Gene: 80380 Human Omim: 605723 Human SwissProt: Q9BQ51 Human |
| 产品图片 |
Sample:
Lane 1: Non-transfected 293T cell lysates
Lane 2: Transfected PD-L2-transfected 293T cell lysates
Primary: Anti-PDCD1LG2 (bsm-51708M) at 1/8000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 40-55 kD
Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
