| 产品编号 | bsm-60006R |
| 英文名称 | Glypican 3 Recombinant Rabbit mAb |
| 中文名称 | 磷脂酰基醇蛋白聚糖-3重组兔单抗 |
| 别 名 | GPC3; glypican3; glypican-3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5; GPC3_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 Flow-Cyt=1:20-100 ICC=1:20-100 IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 65 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Glypican 3: 1-250/580 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Subcellular Location: Cell membrane and Secreted, extracellular space. Tissue Specificity: Highly expressed in lung, liver and kidney. DISEASE: Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Similarity: Belongs to the glypican family. SWISS: P51654 Gene ID: 2719 Database links: Entrez Gene: 2719 Human Entrez Gene: 14734 Mouse Omim: 300037 Human SwissProt: P51654 Human SwissProt: Q8CFZ4 Mouse Unigene: 644108 Human Unigene: 22515 Mouse Unigene: 9717 Rat GPC-3磷脂酰基醇蛋白聚糖-3是一种硫酸类肝素蛋白多糖，GPC-3在不同的组织中具有不同的生物学功能，甚至起着完全不同的作用，可能与肿瘤组织类型、发病机制等密切相关．GPC3在绝大多数HCC(肝细胞癌)患者有表达。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Glypican 3 monoclonal antibody, unconjugated (bsm-60006R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
