| 产品编号 | bsm-60400M |
| 英文名称 | NRF1 Recombinant Mouse mAb |
| 中文名称 | 核呼吸因子-1重组鼠单抗 |
| 别 名 | erythroid derived 2; like 1; Locus control region factor 1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1_HUMAN; NFE2 related factor 1; NFE2-related factor 1; NFE2L1; NRF-1; NRF 1; Nuclear factor; Nuclear factor erythroid 2-related factor 1; Nuclear factor erythroid derived 2 like 1; TCF-11; TCF11; Transcription factor 11; Transcription factor HBZ17; Transcription factor LCR F1; Transcription factor LCR-F1. |
| 研究领域 | 染色质和核信号 生长因子和激素 转录调节因子 |
| 抗体来源 | Mouse |
| 克隆类型 | |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ICC=1:100 IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NRF1 |
| 亚 型 | IgG2b/Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] SWISS: Q16656 Gene ID: 4899 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 54 kDa
Observed MW: 70 kDa
Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti- NRF1 (bsm-60400M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60400M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
