产品编号 | bsm-60236R |
英文名称 | IDO1 Recombinant Rabbit mAb |
中文名称 | 吲哚胺2,3-双加氧酶重组兔单抗 |
别 名 | Indoleamine 2, 3-dioxygenase; 3-dioxygenase; I23O1_HUMAN; IDO 1; IDO; IDO-1; INDO; indolamine 2, 3 dioxygenase; Indole 2 3 dioxygenase; indoleamine 2 3 dioxygenase 1; indoleamine 2 3 dioxygenase; Indoleamine 2, 3-dioxygenase 1; Indoleamine pyrrole 2 3 dioxygenase; Indoleamine-pyrrole 2; indoleamine-pyrrole 2, 3 dioxygenase. |
研究领域 | 神经生物学 信号转导 细胞粘附分子 |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | ID2C1 |
交叉反应 | Human, |
产品应用 | IHC-P=1:200-1:1000 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 45 kDa |
检测分子量 | 45 |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human IDO1 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] SWISS: P14902 Gene ID: 3620 Database links: Entrez Gene: 3620 Human SwissProt: P14902 Human |
产品图片 |
Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60236R
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60236R
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |