| 产品编号 | bsm-60288R |
| 英文名称 | URI1 Recombinant Rabbit mAb |
| 中文名称 | 19号染色体开放阅读框2重组兔单抗 |
| 别 名 | C19orf2; Chromosome 19 open reading frame 2; NNX3; PPP1R19; Protein NNX3; Protein phosphatase 1 regulatory subunit 19; RMP_HUMAN; RNA polymerase II subunit 5 mediating protein; RNA polymerase II subunit 5-mediating protein; RPB5 mediating protein; RPB5-mediating protein; Unconventional prefoldin RPB5 interactor 1; Unconventional prefoldin RPB5 interactor; URI; URI1, prefoldin-like chaperone. |
| 研究领域 | 染色质和核信号 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | U3F5 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IHC-P=1:1000 ICC=1:50 IF=1:50-100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STMN1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011] SWISS: O94763 Gene ID: 8725 Database links: Entrez Gene: 8725 Human SwissProt: O94763 Human |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at
room temperature
Lysate: HeLa, 293T, U87MG
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 60 kDa
Observed MW: 79 kDa
Tissue:Human ovarian carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60288R
Cell line: A431
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60288R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
