| 产品编号 | bsm-54396R |
| 英文名称 | ADH1A Recombinant Rabbit mAb |
| 中文名称 | 乙醇脱氢酶1重组兔单抗 |
| 别 名 | ADH1A_HUMAN; EC:1.1.1.1; Alcohol dehydrogenase 1A; ADH1; Alcohol dehydrogenase subunit alpha; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 14D1 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 ICC=1:50-100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ADH1A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010] Subcellular Location: Cytoplasmic. Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. SWISS: P07327 Gene ID: 124 Database links: Entrez Gene: 124 Human Entrez Gene: 11522 Mouse SwissProt: P07327 Human SwissProt: P00329 Mouse |
| 产品图片 |
Sample:
Lane 1: Rat Liver tissue lysates
Primary: Anti-ADH1A (bsm-54396R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
