产品编号 | bs-39021R |
英文名称 | Phospho-PTPN11 (Tyr511) Rabbit pAb |
中文名称 | 磷酸化蛋白酪氨酸磷酸酶2抗体 |
别 名 | PTN11_HUMAN; Tyrosine-protein phosphatase non-receptor type 11; PTP2C; SHPTP2; EC:3.1.3.48; Protein-tyrosine phosphatase 1D(PTP-1D); Protein-tyrosine phosphatase 2C(PTP-2C); SH-PTP2(SHP-2; Shp2); SH-PTP3; protein tyrosine phosphatase non-receptor type 11; CFC; NS1; JMML; BPTP3; PTP2C; METCDS; |
产品类型 | 磷酸化抗体 |
研究领域 | 肿瘤 免疫学 神经生物学 信号转导 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Pig, Cow, Rabbit, Zebrafish, Sheep, ) |
产品应用 | ELISA=1:5000-10000 IHC-P=1:400-800 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 68 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr511: AQ(p-Y)RF |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non transmembrane PTP, designated SHPTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N terminal to the PTP domain. A second and much more widely expressed PTP with SH2 domains, SHPTP2 (also designated PTP1D and Syp), has been identified. SHP2 is a protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell SWISS: Q06124 Gene ID: 5781 Database links: Entrez Gene: 5781 Human Entrez Gene: 19247 Mouse Omim: 176876 Human SwissProt: Q06124 Human SwissProt: P35235 Mouse Unigene: 506852 Human Unigene: 474046 Mouse Unigene: 8681 Mouse Unigene: 98209 Rat |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PTPN11(Tyr511)) Polyclonal Antibody, Unconjugated (bs-39021R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PTPN11(Tyr511)) Polyclonal Antibody, Unconjugated (bs-39021R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |