| 产品编号 | bsm-60429R |
| 英文名称 | ABCB11 Recombinant Rabbit mAb |
| 中文名称 | 胆汁酸盐输出泵重组兔单抗 |
| 别 名 | ABCBB_HUMAN; Bile salt export pump; BSEP; EC:7.6.2.-; ATP-binding cassette sub-family B member 11; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | G8A8 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:1000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 146 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008] Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. Database links: Entrez Gene: 8647 Human Entrez Gene: 27413 Mouse SwissProt: O95342 Human SwissProt: Q9QY30 Mouse 催化主要的疏水性胆汁盐（例如牛磺酸和甘氨酸缀合的胆酸）以ATP依赖性方式穿过肝细胞的小管膜，因此参与肝胆汁酸的稳态，因此通过调节胆汁脂质的分泌参与脂质的稳态 以胆汁盐依赖的方式。 牛磺酸结合的胆汁盐比甘氨酸结合的胆汁盐更快地运输。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Jurkat, 2: HepG2, 3: A549, 4: LNCaP, 5: Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 146 kDa
Observed MW: 146 kDa
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60429R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
