| 产品编号 | bsm-60456R |
| 英文名称 | GPNMB Recombinant Rabbit mAb |
| 中文名称 | GPNMB重组兔单抗 |
| 别 名 | HGFIN; NMB; PLCA3; DC-HIL; Dchil; ipd; GPNMB_HUMAN; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; GPNMB_MOUSE; Dendritic cell-associated transmembrane protein; Osteoactivin; GPNMB_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | F8D5 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:1000 IHC-P=1:100-500 Flow-Cyt=1:50-1:100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 检测分子量 | 120 |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 该蛋白是I型跨膜糖蛋白，与pMEL17前体（一种黑素细胞特异性蛋白）具有同源性。 GPNMB在低转移性人黑素瘤细胞系和异种移植物中显示表达，但在高转移性细胞系中未显示表达。 GPNMB可能参与生长延迟和转移潜能的降低。 已经为该基因发现了编码不同同工型的两个转录物变体。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with GPNMB monoclonal antibody, unconjugated (bsm-60456R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Cell line: EL4
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60456R
Cell line: A375
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60456R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
