| 产品编号 | bsm-60560R |
| 英文名称 | Fibrillarin Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL_HUMAN; FIB; FIB1; FLRN; Nop1p; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2''-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | H4C3 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:1000-1:3000 ICC=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | S-腺苷-L-甲硫氨酸依赖的甲基转移酶，具有使RNA和蛋白质甲基化的能力。通过催化前核糖体RNA中核糖部分的位点特异性2'-羟基甲基化参与前rRNA加工。位点特异性由与底物碱基配对的引导RNA提供。甲基化发生在与引导RNA碱基配对的序列的特征距离处。可能催化C/D-RNP复合物中U6-snRNAs的2'-O-甲基化。U6-snRNA 2'-O-甲基化是mRNA剪接保真度所必需的。还通过介导组蛋白H2A（H2AQ104me）的“Gln-105”甲基化而充当蛋白质甲基转移酶，这是一种损害FACT复合物结合的修饰，并特异性存在于35S核糖体DNA位点。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:3000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, HepG2, 293T
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 37 kDa
Observed MW: 37 kDa
Cell line: U-2 OS
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60560R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
