| 产品编号 | bsm-60580R |
| 英文名称 | KDM4B Recombinant Rabbit mAb |
| 中文名称 | 赖氨酸特异性脱甲基酶4B重组兔单抗 |
| 别 名 | KDM4B_HUMAN; Lysine-specific demethylase 4B; JHDM3B; JMJD2B; KIAA0876; EC:1.14.11.66; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; [histone H3]-trimethyl-L-lysine(9) demethylase 4B; lysine demethylase 4B; MRD65; TDRD14B |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | G8C10 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-1:1000 Flow-Cyt=1:50-1:100 ICC=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 122 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. Tissue Specificity: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. Database links: Entrez Gene: 23030 Human SwissProt: O94953 Human 赖氨酸脱甲基酶4B（KDM4B）是一种组蛋白去甲基化酶，它能催化H3K9me3的去甲基化反应，松弛染色质结构和允许转录因子进入，即通过表观遗传方式调节基因表达。组蛋白赖氨酸残基的甲基化状态对于基因组激活和非激活区域的形成是一个主要决定因素，并且在发育期间对于基因组的正确编码发挥关键作用。研究发现，KDM4B可能在 ERα 结合位点删除抑制性组蛋白标记，为重塑染色质的酶和转录因子产生锚定位点，进而促进 ERα 介导的转录。此外，过表达KDM4B通过对H3K9me3的去甲基化会导致LINE-1拷贝数、转座活性和DNA损伤程度增加，而KDM4B抑制剂的使用抑制了LINE-1介导的DNA损伤。KDM4B在在乳腺癌、结肠癌、卵巢癌、肺癌和前列腺癌中均有高表达，其致病机制为肿瘤的预防和靶向治疗提供线索。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: T47D, 2: LNCap, 3: K562
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 122 kDa
Observed MW: 150 kDa
Cell line: K562
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60580R
Cell line: K562
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60580R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
