| 产品编号 | bsm-60601R |
| 英文名称 | Laminin B1 Recombinant Rabbit mAb |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | H3F4 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | IHC-P=1:100-500 Flow-Cyt=1:50-1:100 ICC=1:50 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Laminin beta 1: 1720-1786 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | laminin通过高亲和力受体与细胞结合，在胚胎发育过程中通过与其他细胞外基质成分相互作用，介导细胞附着、迁移和组织到组织中。参与大脑皮层层状结构的组织。它可能是基底膜/胶质细胞界限的完整性所必需的，基底膜/胶质细胞界限是放射状胶质细胞末端的锚定点，也是迁移神经元的物理屏障。放射状胶质细胞在大脑皮质发育中起着中心作用，它们既是大脑皮质的增殖单位，又是神经元向软脑膜表面迁移的支架。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
