| 产品编号 | bsm-60609R |
| 英文名称 | CDK8 Recombinant Rabbit mAb |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | B7D5 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:1000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 细胞周期蛋白依赖性激酶8（CDK8）是细胞周期的调控因子，可激活周期素C（Cyclin C），作用于G1/S期，调控正常细胞周期。CDK8连同Cyclin ，Med12和Med13构成中介体复合物，广泛参与RNA聚合酶II和基因特异转录因子间的信息传递，既可激活转录又可抑制转录。此外， CDK8可以激活Wnt/β-catenin和Notch等多种信号转导通路，从而诱导直肠癌，黑色素瘤等恶性肿瘤的发生和转移。最近研究发现，CDK8基因活性的阻断能够在极大程度上降低癌细胞的葡萄糖利用率。 |
| 产品图片 |
Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60609R
Tissue: Mouse colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60609R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
