| 产品编号 | bsm-60555R |
| 英文名称 | SUV39H1 Recombinant Rabbit mAb |
| 中文名称 | 组蛋白H3 K9甲基转移酶重组兔单抗 |
| 别 名 | SUV91_HUMAN; Histone-lysine N-methyltransferase SUV39H1; KMT1A; UV39H; EC:2.1.1.355; Histone H3-K9 methyltransferase 1(H3-K9-HMTase 1); Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1(Su(var)3-9 homolog 1); SUV39H1 histone lysine methyltransferase; MG44; SUV39H; H3-K9-HMTase 1; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | E2B10 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] 组蛋白甲基转移酶，以单乙基化H3‘Lys-9’为底物，特异性地对组蛋白H3的‘Lys-9’进行三甲基化。也有弱甲基化组蛋白H1（体外）。H3‘Lys-9’三甲基化通过向甲基化组蛋白中招募HP1（CBX1、CBX3和/或CBX5）蛋白，代表表观遗传转录抑制的一个特定标记。主要作用于异铬酸盐区，在周中心区和端粒区的本构性异染色质的建立中起着重要作用。H3'Lys-9'三甲基化也需要在中心周重复点直接DNA甲基化。SUV39H1通过与RB1相互作用而以组蛋白H3为靶点，参与了抑制肌D1刺激分化、调控细胞周期退出和进入分化的控制开关、PML-RARA融合蛋白的抑制、BMP诱导的抑制等过程，抑制IgA的开关重组和端粒长度的调节。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, A431, 3T3
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 48 kDa
Observed MW: 48 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
