| 产品编号 | bsm-60464M |
| 英文名称 | MTHFD1 Recombinant Mouse mAb |
| 中文名称 | 亚甲基四氢叶酸脱氢酶单克隆抗体 |
| 别 名 | C1TC_HUMAN; C-1-tetrahydrofolate synthase, cytoplasmic; MTHFC; MTHFD; C1-THF synthase; Epididymis secretory sperm binding protein; C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed; Methylenetetrahydrofolate dehydrogenase; methylenetetrah |
| 研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | |
| 克 隆 号 | F9D9 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:1000 ICC=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 110 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG1/Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Database links: Entrez Gene: 4522 Human Entrez Gene: 108156 Mouse Omim: 172460 Human SwissProt: P11586 Human SwissProt: Q922D8 Mouse |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room
temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L
(HRP)
Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 102 kDa
Observed MW: 102 kDa
Cell line: HEK-293
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60464M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
