| 产品编号 | bsm-60444R |
| 英文名称 | ABCE1 Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | ATP-binding cassette sub-family F(GCN20) member 1; Abc50; GCN20; AU041969; D17Wsu166e; Abcf1; ABC27; ATP binding cassette 50(TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; EST123147; TNF alpha stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667; ABCF1_HUMAN. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | A12F4 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:1000 ICC=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human ABCE1: 574-599 |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 共翻译质量控制因子参与了不通过衰变（NGD）途径。与PELO和HBS1L一起，是80S核糖体形成48S复合物和空位80S核糖体解离所必需的。与PELO和HBS1L一起识别停滞的核糖体并促进组装在不间断mRNAs上的延伸复合物的解离，这触发了mRNA的核内裂解，这是一种释放非功能性核糖体和降解受损mRNAs的机制，是No-Go衰变（NGD）途径的一部分。在mRNA周转的调控中起作用，在线粒体外膜定位mRNA翻译的质量控制中起作用。作为PINK1调控信号的一部分，在线粒体损伤时被CNOT4泛素化；这种修饰产生多泛素信号，将自噬受体招募到线粒体外膜并启动有丝分裂吞噬。RNASEL特异性蛋白抑制剂，拮抗2-5A（5'-磷酸化2'，5'-连接的寡腺苷酸）与RNASEL的结合。干扰素调节的2-5A/RNASEL途径抗病毒作用的负调节因子。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with ABCE1 monoclonal antibody, unconjugated (bsm-60444R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
