| 产品编号 | bsm-60446M |
| 英文名称 | PTPN2 Recombinant Mouse mAb |
| 中文名称 | 酪氨酸蛋白磷酸酶非受体2型单克隆抗体 |
| 别 名 | PTN2_HUMAN; Tyrosine-protein phosphatase non-receptor type 2; PTPT; EC:3.1.3.48; T-cell protein-tyrosine phosphatase(TCPTP); protein tyrosine phosphatase non-receptor type 2; PTN2; TCPTP; TC-PTP; TCELLPTP; |
| 研究领域 | 信号转导 激酶和磷酸酶 |
| 抗体来源 | Mouse |
| 克隆类型 | |
| 克 隆 号 | F3H2 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:1000-1:5000 IHC-P=1:100-500 ICC=1:50 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011] Subcellular Location: [Isoform 2]: Nucleus. Cytoplasm. Cell membrane. Note=Predominantly localizes to chromatin (By similarity). Able to shuttle between the nucleus and the cytoplasm and to dephosphorylate plasma membrane receptors (PubMed:9488479). Recruited by activated ITGA1 at the plasma membrane. {ECO:0000250, ECO:0000269|PubMed:9488479}. Tissue Specificity: Ubiquitously expressed. Isoform 2 is probably the major isoform. Isoform 1 is expressed in T-cells and in placenta. Database links: Entrez Gene: 5771 Human Entrez Gene: 19255 Mouse SwissProt: P17706 Human SwissProt: Q06180 Mouse 去磷酸化受体蛋白酪氨酸激酶的非受体型酪氨酸特异性磷酸酶，包括INSR、EGFR、CSF1R、PDGFR。在细胞核或细胞质中也去磷酸化非受体蛋白酪氨酸激酶，如JAK1、JAK2、JAK3、Src家族激酶、STAT1、STAT3和STAT6。能够负调控许多信号通路和生物过程，如造血，炎症反应，细胞增殖和分化，葡萄糖稳态。在免疫系统的发育中起着多方面的重要作用，通过FYN和LCK的去磷酸化来控制T细胞的分化和活化，在T细胞受体信号转导中发挥作用。去磷酸化CSF1R，负调控其下游信号和巨噬细胞分化。通过细胞质激酶JAK1、JAK3及其底物STAT1的去磷酸化负调控细胞因子（IL2/白介素-2和干扰素）介导的信号传导，这些激酶在细胞因子受体下游传播信号。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PTPN2 monoclonal antibody, unconjugated (bsm-60446M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTPN2 Monoclonal Antibody, Unconjugated(bsm-60446M) at 1:50 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-PTPN2 antibody (bsm-60446M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
