| 产品编号 | bsm-60077M |
| 英文名称 | phospho-Histone H3 (Thr3) Mouse mAb |
| 中文名称 | 磷酸化组蛋白H3单克隆抗体 |
| 别 名 | H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; H3/b; H3C1; H3FB; HIST1H3D; H3.1; H3/c; H3FC; HIST1H3C; H3/d; H3FD; HIST1H3E; H3.f; H3/f; H3FF; HIST1H3I; H3/h; H3FH; HIST1H3G; H3/j; H3FJ; HIST1H3J; H3/k; H3F1K; H3FK; HIST1H3H; H3/l; H3FL; HIST1H3B; H3/i; H3FI; HIST1H3F; H3.2-221; H3c13; H3c14; H3c15; Hist1; H3-143; H3-b; H3-53; H3-f; H3-291; H3-H; H3.1-221; H3 histone; Hist1h2ail; Hist1h2ail1; Hist2h3c; Hist3h3; H31_HUMAN; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3FC HIST1H3C; H33_HUMAN; H3-3A; H3-3B; H3.3A; H3F3; H3F3A; H3.3B; H3F3B; H31T_HUMAN; H3-4; H3/t; H3t; H3/g; Histone H3.4; H3FT; H3C_HUMAN; H3-5; Histone H3.5; H3F3C; H32_HUMAN; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; Histone H3/m; Histone H3/o; HIST2H3A; H3F2; H3FM; HIST2H3D; H31_MOUSE; H3a; H3g; H3.1-291; H3h; H3.1-I; H3i; H32_MOUSE; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3.2; H3b; H3f; H3.2-616; Hist2h3b; H3.2-615; Hist2h3c1; Hist2h3ca1; H3.2-614; Hist2h3c2; Hist2h3ca2; H31_RAT; H3 clustered histone 1; H3 histone family, member A; histone 1, H3a; histone cluster 1, H3a; histone cluster 1 H3 family member a; H3C1 | Histone H3 (phospho-T3); H3T3P; p-Histone H3; phospho-Histone H3; p-H3; H3C1 | Histone H3 (phospho-Thr3) |
| 产品类型 | 磷酸化抗体 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | E7C4 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 检测分子量 | 17 |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Antigen affinity purification |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白翻译后修饰（PTMs）是表观遗传学调控染色质结构的关键机制，被称为“组蛋白密码”。组蛋白上的翻译后修饰包括乙酰化，甲基化，磷酸化和近年发现的一些新型酰化修饰。这些组蛋白修饰直接影响染色质和转录因子或其他表观调控子的结合，改变基因组的稳定性和基因转录等。组蛋白磷酸化通常发生在核心组蛋白N端的丝氨酸，苏氨酸或酪氨酸上，在DNA修复，转录和染色质折叠等方面起着重要作用。广为人知的组蛋白磷酸化位点为H2A.xS139ph，有文献报道此位点与DNA损伤相关。组蛋白磷酸化主要参与有丝分裂和减数分裂过程。参与调控磷酸化水平的激酶与磷酸酶多种多样。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
