| 产品编号 | bsm-60085M |
| 英文名称 | Di/Tri-Methyl-Histone H4 (Lys20) Recombinant Mouse mAb |
| 中文名称 | 二/三甲基化组蛋白H4K20重组小鼠单抗 |
| 别 名 | H4-16; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; HIST4H4; H4/o; H4C16; HIST2H4B; H4/m; H4FM; H4M; HIST1H4I; TEBIVANED4; TEVANED4; H4FA; HIST1H4A; H4/b; H4FB; HIST1H4D; dJ221C16.9; H4; H4/c; H4FC; HIST1H4F; H4/d; H4F2iii; H4FD; HIST1H4K; dJ160A22.1; H4/e; H4F2iv; H4FE; HIST1H4J; TEBIVANED2; TEVANED2; dJ160A22.2; H4/g; H4FG; HIST1H4C; TEBIVANED1; TEVANED1; dJ221C16.1; H4/h; H4FH; HIST1H4H; H4/I; H4FI; HIST1H4B; H4/j; H4FJ; HIST1H4E; TEBIVANED3; TEVANED3; H4.k; H4/k; H4FK; HIST1H4L; FO108; H4/n; H4F2; H4FN; HIST2H4; HIST2H4A; Gm11275; H4f16; Hist1h4m; Hist1h4n; B130044J01Rik; 1700024H08Rik; H2c8; H4_DROME; His4; His4r; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; H4r; H4_HUMAN; H4/A; H4FO; H4_MOUSE; H4-53; H4-12; H4_SCHPO; hhf1; hhf2; hhf3; h4.1; h4.2; h4.3; H4 clustered histone 1; H4 histone family, member A; histone 1, H4a; histone cluster 1, H4a; histone cluster 1 H4 family member a; H4C1 | Histone H4 (di/tri-methyl-K20); H4K20me2/me3; me-Histone H4; methyl-Histone H4; me-H4; H4C1 | Histone H4 (di/tri-methyl-Lys20) |
| 产品类型 | 甲基化抗体 |
| 抗体来源 | Mouse |
| 克隆类型 | |
| 克 隆 号 | G9B11 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 检测分子量 | 11 |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 蛋白质的ε-氨基赖氨酸乙酰化是控制蛋白质活性的一种重要的可逆修饰。核心组蛋白的氨基末端在多个位点发生赖氨酸甲基化，称为“组蛋白密码”或“表观遗传密码”。核心组蛋白中赖氨酸甲基化是形成基因组活性区和非活性区的主要决定因素，因此在多种细胞事件中起着至关重要的作用。在大多数物种中，赖氨酸甲基化主要发生在组蛋白H3（Lys4、9、27、36、79）和H4（Lys20）上，并且与转录激活和沉默有关。受组蛋白甲基化酶（HMTs）和组蛋白去甲基化酶（HDMs）调控的组蛋白甲基化在癌症和其他疾病的病理过程中受到损害，因此，调节组蛋白赖氨酸甲基化的酶已成为抗癌药物的靶点。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidney, rH4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60085M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
