| 产品编号 | bsm-60591R |
| 英文名称 | PAX9 Recombinant Rabbit mAb |
| 中文名称 | 配对盒基因9重组兔单抗 |
| 别 名 | Paired box 9; Paired box gene 9; Paired box homeotic gene 9; Paired box protein 9; Paired box protein Pax 9; Paired box protein Pax9; Paired domain gene 9; PAX 9; PAX9_HUMAN. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | G12B2 |
| 交叉反应 | Human, (predicted: Rat, ) |
| 产品应用 | WB=1:500-1:1000 IHC-P=1:50-1:100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. Mice lacking this gene exhibit impaired development of organs, musculature and the skeleton, including absent and abnormally developed teeth, and neonatal lethality. Mutations in the human gene are associated with selective tooth agenesis-3. [provided by RefSeq, Sep 2015] PAX9是一种在人类体内由PAX9基因编码的蛋白质。该基因是转录因子PAX家族中的一员。在小鼠胚胎发生过程中，PAX9从胚胎期第8.5天开始表达，到胚胎期第9.5天时更加明显；在这个阶段，它的表达仅限于咽内胚层。随后，PAX9逐渐在轴向骨架中表达。PAX9是颅面、牙齿和肢体发育所必需的，也可能涉及到鳞状上皮以及各种器官及骨骼的发育。在某些人群中，PAX9在智齿缺失中发挥了一定的作用。同时，它也是胸腺、甲状旁腺、鳃下体、牙齿、颅骨和喉部的骨骼以及远端肢体正常发育所必需的转录因子。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PAX9 monoclonal antibody, unconjugated (bsm-60591R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PAX9) monoclonal Antibody, unconjugated (bsm-60591R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
