产品编号 | bsm-60206R |
英文名称 | β-Hydroxybutyryl-Histone H3 (Lys9) Recombinant Rabbit mAb |
中文名称 | |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | E6A5 |
交叉反应 | (predicted: Human, Rat, ) |
产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | kDa |
检测分子量 | |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 | 组蛋白能被多种酶催化发生修饰,比如乙酰化,甲基化,磷酸化和泛素化等。赖氨酸β-羟基丁酰化(Kbhb)是近期发现的一种主要富集在活性基因启动子区的组蛋白标记。 研究表明,在培养细胞时,提高β-羟基丁酸盐浓度;长期禁食或用链脲佐菌素诱导小鼠糖尿病酮症,可显著提高细胞或小鼠肝脏中的Kbhb水平。 组蛋白Kbhb是一种可以将代谢与基因表达偶联的全新表观遗传调控机制,为研究染色质调控以及一些重要生理病理疾病比如糖尿病,癫痫和肿瘤中β-羟基丁酸盐的多种功能提供了新途径。 |
产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium 3-ohbutyrylate (50mM, 72hr)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Rat colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60206R
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |