| 产品编号 | bsm-60623R |
| 英文名称 | CGA Recombinant Rabbit mAb |
| 中文名称 | 促甲状腺激素α重组兔单抗 |
| 别 名 | GLHA_HUMAN; Glycoprotein hormones alpha chain; Anterior pituitary glycoprotein hormones common subunit alpha; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; Choriogonadotropin alpha chain; Chorionic gonadotrophin subunit alpha(CG-alpha); Follicle-stimulating hormone alpha chain(FSH-alpha); Follitropin alpha chain; Luteinizing hormone alpha chain(LSH-alpha); Lutropin alpha chain; Thyroid-stimulating hormone alpha chain(TSH-alpha); Thyrotropin alpha chain; glycoprotein hormones, alpha polypeptide; HCG alpha; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | D4A7 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin. Subcellular Location: Secreted. Database links: Entrez Gene: 1081 Human SwissProt: P01215 Human 活性异二聚体糖蛋白激素促甲状腺素/促甲状腺激素/TSH、促黄体激素/促黄体生成素/LH、促卵泡素/促卵泡激素/FSH和绒毛膜促性腺激素/CG的共享α链。这些激素结合靶细胞上的特定受体，进而激活下游信号通路。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CGA monoclonal antibody, unconjugated (bsm-60623R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60623R
Tissue: Human kidney (Negative tissue)
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60623R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
