| 产品编号 | bsm-60432R |
| 英文名称 | TSHB Recombinant Rabbit mAb |
| 中文名称 | 促甲状腺素β亚单位重组兔单抗 |
| 别 名 | TSHB_HUMAN; Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta(TSH-B; TSH-beta); Thyrotropin beta chain; Thyrotropin alfa; TSH-β; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | D7C6 |
| 交叉反应 | (predicted: Human, Rat, ) |
| 产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 Flow-Cyt=1:50-1:100 ICC=1:50 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Subcellular Location: Secreted. Database links: Entrez Gene: 7252 Human
SwissProt: P01222 Human TSHB（甲状腺刺激激素亚基Beta）是一种蛋白质编码基因。 与TSHB相关的疾病包括甲状腺功能减退，先天性，非甲状腺功能减退4和中枢性先天性甲状腺功能减退。其相关途径包括通过GPCR进行信号传递和肽激素代谢。与该基因相关的基因本体论（GO）注释包括激素活性。该基因的重要旁系同源物是FSHB。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Rat pituitary
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 16 kDa
Observed MW: 16 kDa
Tissue: Rat pituitary
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60432R
Cell line: U-2 OS
Fixative: 100% methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60432R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60432R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
