| 产品编号 | bsm-60433R |
| 英文名称 | CLDN1 Recombinant Rabbit mAb |
| 中文名称 | 紧密连接蛋白1重组兔单抗 |
| 别 名 | CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | B5D10 |
| 交叉反应 | (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Claudin 1: 180-211 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008] Subcellular Location: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed, with highest levels in liver and kidney. Database links: Entrez Gene: 9076 Human Entrez Gene: 12737 Mouse SwissProt: O95832 Human SwissProt: O88551 Mouse claudins充当紧密连接复合物的主要成分，该紧密连接复合物调节上皮的通透性。尽管某些claudin家族成员在不可渗透屏障的形成中起着至关重要的作用，但其他一些介导了对离子和小分子的渗透性。通常，几个claudin家族成员会共表达并相互影响，这决定了总体渗透性。 CLDN1是必需的，可防止小分子通过表皮中的紧密连接发生旁细胞扩散，而CLDN1是皮肤的正常屏障功能所必需的。正常的水体内平衡和防止水通过皮肤过多流失可能是必需的，这可能是通过间接影响其他蛋白质的表达水平来实现的，因为CLDN1本身似乎对角质形成细胞紧密连接中的水屏障形成不可或缺。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 monoclonal antibody, unconjugated (bsm-60433R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 monoclonal antibody, unconjugated (bsm-60433R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
