| 产品编号 | bsm-60262M |
| 英文名称 | PPP3CB Mouse mAb |
| 中文名称 | |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | F4G2 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-1:1000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 丝氨酸/苏氨酸蛋白磷酸酶2B催化亚基亚型(PP2BB)是一种在人体内由PPP3CB基因编码的酶。这个亚基可能在钙调蛋白的激活中起作用。它是一种钙调素刺激的蛋白磷酸酶，在细胞内Ca2+信号的转导中起重要作用。它能去磷酸化激活转录因子NFATC1，去磷酸化激活转录因子ELK1。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 59 kDa
Observed MW: 59 kDa
Tissue: Human brain
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60262M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
