| 产品编号 | bsm-60214M |
| 英文名称 | Histone H4 (NT) Mouse mAb |
| 中文名称 | 组蛋白H4单克隆抗体 |
| 别 名 | H4-16; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; HIST4H4; H4/o; H4C16; HIST2H4B; H4/m; H4FM; H4M; HIST1H4I; TEBIVANED4; TEVANED4; H4FA; HIST1H4A; H4/b; H4FB; HIST1H4D; dJ221C16.9; H4; H4/c; H4FC; HIST1H4F; H4 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | D12G5 |
| 交叉反应 | (predicted: Human, Mouse, Rat, Monkey, ) |
| 产品应用 | WB=1:500-1:2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Antigen affinity purification |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 核小体由四个核心组蛋白（H2A、H2B、H3和H4）组成，是染色质的主要组成部分。核小体将DNA包裹并压缩成染色质，限制DNA进入细胞机制。组蛋白H4是参与真核细胞染色质结构的五种主要组蛋白之一。H4具有一个主要的球状结构域和一个长的N末端尾巴，它参与了“串珠”组织的核小体结构。组蛋白是高度翻译后修饰的。共价键合修饰包括N端尾部的乙酰化和甲基化。这些修饰可能改变位于与其亲本组蛋白八聚体相关的DNA上的基因的表达。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: MCF-7, BRL, N2a, COS-7, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60214M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
