| 产品编号 | bsm-30162A |
| 英文名称 | mouse CD86 Rat mAb |
| 中文名称 | 小鼠CD86单克隆抗体 |
| 别 名 | B7-2; B7.2; B70; CD28LG2; LAB72; CD86_HUMAN; CD86; Activation B7-2 antigen; BU63; CTLA-4 counter-receptor B7.2; FUN-1; |
| 抗体来源 | Rat |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3D5 |
| 交叉反应 | Human, (predicted: Mouse, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | 72-74 |
| 性 状 | Liquid |
| 亚 型 | Rat IgG2a, k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with mouse CD86 monoclonal antibody, unconjugated (bsm-30162A) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
scatter diagram showing mouse spleen stained with CD86.The cells were incubated with the antibody (bsm-30162A, 1 µg/Test) for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.Acquisition of >20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
