| 产品编号 | bsm-60633R |
| 英文名称 | JunB Recombinant Rabbit mAb |
| 中文名称 | 活化蛋白激酶B重组兔单抗 |
| 别 名 | JUNB_HUMAN; Transcription factor JunB; Transcription factor jun B; Transcription factor AP-1 subunit JunB; Jun B; |
| 研究领域 | 肿瘤 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human JunB |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'. SWISS: P17275 Gene ID: 3726 Database links: Entrez Gene: 3726 Human Entrez Gene: 16477 Mouse SwissProt: P17275 Human SwissProt: P09450 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse Uterus tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human MCF-7 cell lysates
Lane 6: Human A431 cell lysates
Primary: Anti-JunB (bsm-60633R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 46,42 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
