| 产品编号 | bsm-60636R |
| 英文名称 | FAP Recombinant Rabbit mAb |
| 中文名称 | 成纤维细胞激活蛋白α重组兔单抗 |
| 别 名 | Prolyl endopeptidase FAP; FAPA; Fibroblast activation protein, alpha; 170 kDa melanoma membrane bound gelatinase; 170 kDa melanoma membrane-bound gelatinase; DPPIV; Fibroblast activation protein alpha; Integral membrane serine protease; SEPR_HUMAN; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; FAPalpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase; Antiplasmin-cleaving enzyme FAP, soluble form; APCE. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 8F2 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 88 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FAP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] SWISS: Q12884 Gene ID: 2191 Database links: Entrez Gene: 2191 Human Entrez Gene: 14089 Mouse Omim: 600403 Human SwissProt: Q12884 Human SwissProt: P97321 Mouse Unigene: 654370 Human Unigene: 41816 Mouse |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with FAP monoclonal antibody, unconjugated (bsm-60636R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
