| 产品编号 | bs-41387R |
| 英文名称 | PRRSV N Rabbit pAb |
| 中文名称 | 猪蓝耳病病毒N蛋白抗体 |
| 别 名 | PRRSV N protein; N protein [Porcine reproductive and respiratory syndrome virus]; nucleocapsid protein; |
| 研究领域 | 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13.8 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant PRRSV N protein: 1-128/128 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. Subclinical infections are common, with clinical signs occurring sporadically in a herd. Clinical signs include reproductive failure in sows such as abortions and giving birth to stillborn or mummified fetuses, and cyanosis of the ear and vulva. In neonatal pigs, the disease causes respiratory distress, with increased susceptibility to respiratory infections such as Glasser's disease. |
| 产品图片 |
Sample:
Lane 1: Recombinant PRRSV N protein, N-His(bs-41387P)
Primary: Anti-PRRSV N (bs-41387R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13.8 kDa
Observed band size: 17 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Pig PRRSV protein, His Tag (Cat. bs-41387P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Pig PRRSV Antibody, the EC50 is 400.8 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
