| 产品编号 | bsm-60654R |
| 英文名称 | phospho-SIRT1 (Ser27) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化沉默调节蛋白1重组兔单抗 |
| 别 名 | SIR2L1; NAD-dependent protein deacetylase sirtuin-1; EC:2.3.1.286; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1(hSIR2); SirtT1 75 kDa fragment; SIR1_HUMAN; |
| 研究领域 | 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | R4C3 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-1:100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 81 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human phospho-SIRT1 (Ser27) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] Subcellular Location: Nucleus, PML body. Cytoplasm. Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm. SirtT1 75 kDa fragment: Cytoplasm. Mitochondrion. Tissue Specificity: Widely expressed. SWISS: Q96EB6 Gene ID: 23411 Database links:
Entrez Gene: 23411 Human Entrez Gene: 93759 Mouse SwissProt: Q96EB6 Human SwissProt: Q923E4 Mouse
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| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1-2: HEK-293+CA (-) (+), 3-4:
Jurkat+CA(-) (+)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 82 kDa
Observed MW: 120 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
