| 产品编号 | bsm-60679R |
| 英文名称 | phospho-BRD2 (Ser37) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化BRD2蛋白重组兔单抗 |
| 别 名 | Bromodomain-containing protein 2; KIAA9001; RING3; O27.1.1; Really interesting new gene 3 protein; BRD2_HUMAN; |
| 产品类型 | 磷酸化抗体 重组兔单抗 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | R1A12 |
| 产品应用 | WB=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 88 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human phospho-BRD2 (Ser37) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010] Subcellular Location: Nucleus. SWISS: P25440 Gene ID: 6046 Database links: Entrez Gene: 6046 Human SwissProt: P25440 Human |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:20000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HEK-293T, 2: HEK-293T + Calyculin
A (100 nM, 60 min)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 88 kDa
Observed MW: 110 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
